Pegylated Recombinant Human Interferon alfa2b

Detailed Product Description

Description:
Recombinant Human Interferon alpha-2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. Pegylated IFN-alpha 2b is manufactured by attaching a 20KD mPEG to the primary amino acid of IFN-alfa 2b.
Quality Control:
Biological Activity: Pegylated IFN-alfa 2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5 x 107 IU/mg.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro.
Endotoxin: Less than 0.1 ng/礸 (IEU/礸) of IFN-alpha 2b.